LLS ROWIAK LaserLabSolutions Newsletter

2015-08-02


Newsletter 2/2015

Dear Reader,

please find below the following topics:

  1. TRAP enzyme histochemistry for Osteoclast detection on bone sections now established with TissueSurgeon
  2. Movie describing Lasermicrotomy
  3. LLS ROWIAK LaserLabSolutions at NSH 2015

TRAP enzyme histochemistry for Osteoclast detection on bone sections now established with TissueSurgeon

The detection of osteoclasts in bone section is not easy, as the cells do not appear in large numbers. They are multinucleated (up to 10 nuclei) and can be found on the bone surface in resorption bays, also called Howships lacunae. A very established way of osteoclast detection is TRAP staining (Tartrate Resistant Acid Phosphatase) which actually detects acid phosphatase enzyme vesicles inside the ostecoclasts. This method is used on either decalcified paraffin sections or ground sections.

For the first time TRAP staining method was proofed on non-decalcified thin sections performed with the Lasermicrotome TissueSurgeon at 10µm thickness. In comparison to decalcified paraffin sections, also hard tissue information is still available for analysis; compared to ground sections, laser sections are much thinner and therefore accessibility to microscopy is improved. In ground sections osteoclasts may be covered by other cell layer.

TRAP stain for osteoclasts (arrow) in rat femur. Staining was performed by Institute of Immunology, University Medicine of Rostock.

Preparation of thin sections is much faster and easier to perform with the laser compared to ground sectioning or paraffin sectioning. The establishment of TRAP enzyme histochemistry on thin sections cut with the lasermicotome TissueSurgeon demonstrates that the laser cutting does not destroy the enzyme function and therefore is suitable for enzyme histochemical analysis.



Movie describing Lasermicrotomy

LLS ROWIAK has produced a little movie “Fast and Easy Production of Histological Sections by Ultrafast Laser Processing??? showing the process of laser microtomy using the TissueSurgeon. The movie can be watched at YouTube.


LLS ROWIAK LaserLabSolutions at NSH 2015

LLS ROWIAK LaserLabSolutions will participate at the 41st NSH Annual Symposium / Convention at the Gaylord National Resort & Convention Center in Washington from August 28-September 2, 2015. Dr. Heiko Richter will hold two workshops:

  • WS# 55 Economic Efficiency of Modern Laser Based Plastic Microtomy

For additional information and registration, please follow this link.

  • ET# 6 Lasermicrotome TissueSurgeon - Contact Free Microtomy

For additional information and registration, please follow this link.

 

We are looking forward to meet you.


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LLS ROWIAK LaserLabSolutions GmbH, Garbsener Landstraße 10, D-30419 Hannover, HRB 210486 , Phone: +49 511 277 2976, Fax: +49 511 277 1766