Publications
Read more about laser based histology, laser microtomy, and laser dissection & imaging
Bächinger, D., Peyton, B., Neubauer, J., Dharmarajan, A., Zhu, M., O‘Malley, J. T., Kallupurackal, V., Senese, S., Brown, A., Wunderlin, S., Kreutzer, S., Weiss, N. M., Richter, H., Dalbert, A., Röösli, C., Kipar, A., Varga, Z., von Rechenberg, B., Amr, S. S. and Eckhard, A. (2025). Integrated Histology and Molecular Profiling of Postmortem Human Auditory and Vestibular Organs via a Poly(Methyl Methacrylate)-Based Workflow. bioRxiv, https://doi.org/10.1101/2025.06.04.657716
Schwab, A., Veldman, J., Kops, N., den Dekker, X., Hiscock, K., van IJcken, W., Sinram, M., Stolze, B., Richter, H., Bertrand, J., Farrell, E., & van Osch, G. J. (2025). RNA sequencing of chondrocytes isolated from the calcified cartilage layer of osteoarthritic osteochondral tissue. Orthopaedic Proceedings, 107-B(SUPP_7), 111-111. https://doi.org/10.1302/1358-992X.2025.7.111
Kotlarz, M.; O’Keeffe, C.; Kronemberger, G.; Burdis, R.; Rana, S.; Rodriguez, N.; Bonizzi, M.; Brama, P.A.J.; Kelly, D. (2025) Biofabrication and in vivo evaluation of a hybrid osteochondral implant consisting of structurally organised engineered cartilage on a 3D-printed metal scaffold. Biomaterials. 327. 123788. https://doi.org/10.1016/j.biomaterials.2025.123788.
MoreSystermans, S.; Ronsmans, C.; Nolens, G.; Meyns, J.; Gilon, Y.; Cobraiville, E. (2025) Dental Implant Osseointegration Following Mandibular Augmentation Using 3D Printed Hydroxyapatite Blocks. An Experimental In Vivo Study. Clinical oral implants research. https://doi.org/10.1111/clr.70056
Szűcs-Takács, Z.; Varga, V.; Bán, F.; Harcsa, V.; Pinke, B.; Várdai, R.; Gajnut, F.; Major, E.; Hornyák, I. Optimization of Gelatin-Based Scaffolds for Soft Tissue Regeneration: In Vitro and In Vivo Performance. Int. J. Mol. Sci. 2025, 26, 9106. https://doi.org/10.3390/ijms26189106
P. Nolte and C. J. Groger, C. Frey, H. Richter, F. Will, T. Bauerle, A. F. Schilling, F. Alves, C. Rusmann, C. Dullin (2025) Automated targeted sectioning of resin-embedded hard tissue specimen using micro-computed tomography in combination with laser microtomy. IEEE Transactions on Biomedical Engineering, doi: 10.1109/TBME.2025.3528739
Lisa Leyssens, Walid El Aazmani, Tim Balcaen, Pascal J. Jacques, Sadrine Horman, Jeremy Goldman, Greet Kerckhofs, (2024) MicroCT and contrast-enhanced microCT to study the in vivo degradation behavior and biocompatibility of candidate metallic intravascular stent materials. https://doi.org/10.1016/j.actbio.2024.11.017
Jonathan Cortese, Géraud Forestier, Sylvia M Bardet, Marie-Laure Perrin, Maxime Baudouin, Alexis Belgacem, Romain Chauvet, Voahirana Ratsimbazafy, Gregory Sasselina, Daphnée Chandellier, Jérémy Mounier, Claude Couquet, Florence Bosselut, Laurent Spelle, Charbel Mounayer, Faraj Terro, Aymeric Rouchaud (2024) Preclinical in vitro and in vivo results of the new silk vista flow diverter with P8RI coating . J NeuroIntervent Surg 2024;0:1–7. doi:10.1136/jnis-2024-021694
Agata Szczodra, Amel Houaoui, Rémy Agniel, Ludovic Sicard, Susanna Miettinen, Jonathan Massera, Caroline Gorin (2024) Boron substitution in silicate bioactive glass scaffolds to enhance bone differentiation and regeneration, Acta Biomaterialia, ISSN 1742-7061, https://doi.org/10.1016/j.actbio.2024.07.053.
Robin Evrard, Julie Manon, Louis Maistriaux, Lies Fieve, Tom Darius, Olivier Cornu, Benoit Lengele, Thomas Schubert (2024) Enhancing the biological integration of massive bone allografts: A porcine preclinical in vivo pilot-study. Bone (in press) https://doi.org/10.1016/j.bone.2024.117213
D. Su, S. Swearson, S. Eliason, K.G. Rice, B.A. Amendt (2024) RNA Technology to Regenerate and Repair Alveolar Bone Defects. J. Dental Res. 103 (6) https://doi.org/10.1177/00220345241242
Leon Riehakainena, Eduarda Mota-Silvaa, Claudia Kusmic, Daniele Panetta, Debora Petroni, Davide Fragnito, Stefano Salvadori, Luca Menichetti (2024) Assessment of tissue response in vivo: PET-CT imaging of titanium and biodegradable magnesium implants. Acta Biomaterialia 184, 461-472
Varga V.; Smeller L.; Várdai R.; Kocsis B.; Zsoldos I.; Cruciani S.; Pala R.; Hornyák I.; Water-Insoluble, Thermostable, Crosslinked Gelatin Matrix for Soft Tissue Implant Development. Int. J. Mol. Sci.2024, 25(8), 4336; https://doi.org/10.3390/ijms25084336
Kamila Iskhakova, D. C. Florian Wieland, Romy Marek, Uwe Y. Schwarze, Anton Davydok, Hanna Cwieka, Tamadur AlBaraghtheh, Jan Reimers, Birte Hindenlang, Sandra Sefa, André Lopes Marinho, Regine Willumeit-Römer, Berit Zeller-Plumhoff (2024) Sheep Bone Ultrastructure Analyses Reveal Differences in Bone Maturation around Mg-Based and Ti Implants. J Funct Biomater. 15(7) 192
Edin Nevzati, Jeannine Rey, Alexander Spiessberger, Manuel Moser, Michel Roethlisberger, Basil Erwin Grüter, Hans Rudolf Widmer, Daniel Coluccia, Serge Marbacher (2024) Aneurysm healing following treatment with biodegradable embolization materials: assessment in a rat sidewall aneurysm model. J. Neurointerventional Surgery https://doi.org/10.1136/jnis-2023-021260
Yustina M. Puspitasari, Stefano Ministrini, Luca Liberale, Ana Vukolic, Philine Baumann-Zumstein, Erik W. Holy, Fabrizio Montecucco, Thomas F. Lüscher, Giovanni G.Camici (2023) Antibody-mediated PCSK9 neutralization worsens outcome after bare-metal stent implantation in mice. Vascular Pharmacology 153, 107170
Nolte, P.; Brettmacher M.; Gröger, C. J.; Gellhaus, T.; Svetlove A.; Schilling, A. F.; Alves, A.; Rußmann, C.; Dullin, C.; (2023) Spatial correlation of 2D hard‑tissue histology with 3D microCT scans through 3D printed phantoms. Sci Rep 13, 18479
Lore Kerkhofs, Anastasiya Starovoyt, Jan Wouters, Tristan Putzeys, Nicolas Verhaert (2023) Optical Coherence Tomography-Based Atlas of the Human Cochlear Hook Region. J. Clin. Med. 12(1), 238; https://doi.org/10.3390/jcm12010238
Diana C. Martinez, Anna Dobkowska, Romy Marek, Hanna Cwieka, Jakub Jaroszewicz, Tomasz Płocinski, Crtomir Donik , Heike Helmholz, Berengere Luthringer-Feyerabend, Berit Zeller-Plumhoff, Regine Willumeit-Romer, Wojciech Swięszkowski (2023) In vitro and in vivo degradation behavior of Mg-0.45Zn-0.45Ca (ZX00) screws for orthopedic applications. Bioactive Materials 28, 132–15
Kevin Janot, Grégoire Boulouis, Géraud Forestier, Fouzi Bala, Jonathan Cortese, Zoltán Szatmáry, Sylvia M. Bardet, Maxime Baudouin, Marie-Laure Perrin, Jérémy Mounier, Claude Couquet, Catherine Yardin, Guillaume Segonds, Nicolas Dubois, Alexandra Martinez, Pierre-Louis Lesage, Yong-Hong Ding, Ramanathan Kadirvel, Daying Dai, Charbel Mounayer, Faraj Terro, Aymeric Rouchaud (2023) WEB shape modifications: angiography–histopathology correlations in rabbits J NeuroIntervent Surg 2023;0:1–7.
Géraud FORESTIER, Jonathan CORTESE, Sylvia M. BARDET, Maxime BAUDOUIN, Kévin JANOT, Voahirana RATSIMBAZAFY, Marie-Laure PERRIN, Jérémy MOUNIER, Claude COUQUET, Catherine YARDIN, Yan LARRAGNEGUY, Flavie SOUHAUT, Romain CHAUVET, Alexis BELGACEM, Sonia BRISCHOUX, Julien MAGNE, Charbel MOUNAYER, Faraj TERRO, Aymeric ROUCHAUD (2023) Comparison of Arterial Wall Integration of different Flow Diverters in rabbits: the CICAFLOW study Journal of Neuroradiology, In press.
Matthias Schlund, Julien Dartus, Sarah Defrançois, Joël Ferri, Jérôme Delattre, Nicolas Blanchemain, Patrice Woisel, Joël Lyskawa, Feng Chai (2023) In vitro and in vivo evaluation of a bio-inspired adhesive for bone fixation. Pharmaceutics 15, 1233
Lucas T. Duong, Stéphane Petit, Stéphane Kerner, Mélodie M. Clerc, Christophe Arnoult, Nunthawan Nowwarote, Thanaphum Osathanon,·Benjamin P.J. Fournier, Juliane Isaac, François C. Ferré (2023) Role of periosteum during healing of alveolar critical size bone defects in the mandible: a pilot study. Clinical Oral Investigations https://doi.org/10.1007/s00784-023-05079-y
Stocchero, Michele; Jinno, Yohei; Toia, Marco; Ahmad, Marianne; Galli, Silvia; Papia, Evaggelia; Herath, Manjula; Becktor, Jonas P (2023) Effect of Drilling Preparation on Immediately Loaded Implants: An In Vivo Study in Sheep. International Journal of Oral & Maxillofacial Implants 38 (3), 607
Martin Rauber, MD; Philipp Nicol, MD; Emina Sabic, MD; Michael Joner, MD, PhD; Marko Noc, MD, PhD (2022) Timing and predictors of definite stent thrombosis in comatose survivors of out-of-hospital cardiac arrest undergoing percutaneous coronary intervention and therapeutic hypothermia (ST-OHCA study). EuroIntervention 18(9), 740–748.
Anastasiya Starovoyt, Bryden C. Quirk, Tristan Putzeys, Greet Kerckhofs, Johan Nuyts, JanWouters, RobertA. McLaughlin, NicolasVerhaert (2022) An optically‑guided cochlear implant sheath for real‑time monitoring of electrode insertion into the human cochlea. Scientifc Reports 12, 19234
Nilton Salles Rosa Neto, Daniel Englert, William H. McAlister, Steven Mumm, David Mills, Deborah J. Veis, Alan Burshell, Alan Boyde, Michael P. Whyte (2022) Periarticular calcifications containing giant pseudo-crystals of francolite in skeletal fluorosis from 1,1-difluoroethane “huffing” Bone 160 (2022) 116421
Diana Krüger, Silvia Galli, Berit Zeller-Plumhoff,D.C., Florian Wieland, Niccolò Peruzzi, Björn Wiese, Philipp Heuser, Julian Moosmann, Ann Wennerberg, Regine Willumeit-Römer (2022) High-resolution ex vivo analysis of the degradation and osseointegration of Mg-xGd implant screws in 3D. Bioactive Materials 13, 37-52
Bouyer M; Garot C; Machillot P; Vollaire J; Fitzpatrick V; Morand S; Boutonnat J; Josserand V; Bettega G; Picart C (2021) 3D-printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect Materials Today Bio 11 (2021) 100113
Luigi Canullo, David Penarrocha Oltra, Paolo Pesce, Cristina Zarauz, Rossano Lattanzio, Miguel Penarrocha Diago, Giovanna Iezzi (2021) Soft tissue integration of different abutment surfaces: An experimental study with histological analysis. Clinical Oral Implants Res. 32 (8), 928-940
Clemens Hufnagl, Erasmia Broussalis, Christophe Cognard, Jochen Grimm, Constantin Hecker, Andreas Oellerer, Muhammed Abdallah, Christoph J Griessenauer, Monika Killer-Oberpfalzer (2021) Evaluation of a novel flow diverter, the DiVeRt system, in an animal model. J NeuroIntervent Surg 0, 1–7. doi:10.1136/neurintsurg-2021-017430
Boyde A (2021) The Bone Cartilage Interface and Osteoarthritis. Calcified Tissue International https://doi.org/10.1007/s00223-021-00866-9
Verhaegen C, Kautbally S, Zapareto D C, Brusa D, Courtoy G, Aydin S, Bouzin C, Oury C, Bertrand L, Jacques P J, Beauloye C, Horman S, Kefer J (2020) Early thrombogenicity of coronary stents: comparison of bioresorbable polymer sirolimus-eluting and bare metal stents in an aortic rat model. Am J Cardiovasc Dis. 10(2):72-83
Alain Hoornaert, Luciano Vidal, Romain Besnier, Jean-Francois Morlock, Guy Louarn, Pierre Layrolle (2020) Biocompatibility and osseointegration of nanostructured titanium dental implants in minipigs. Clinical Oral Implants Res. 31 (6), 526-535
Julia Latza, Maresa Otte, Tobias Lindner, Dagmar-Christiane Fischer, Sven Bruhn, Robin Hollinski, Mareike Warkentin, Thomas Mittlmeier, Brigitte Müller-Hilke (2020) Interval Training Is Not Superior to Endurance Training With Respect to Bone Accrual of Ovariectomized Mice. Front. Physiol. 09, Sec. Exercise Physiology 11, https://doi.org/10.3389/fphys.2020.01096
Zeller-Plumhoff B, Malicha C, Krüger D, Campbella G, Wiesea B, Galli S, Wennerberg A, Willumeit-Römer R, Wieland F (2020) Analysis of the bone ultrastructure around biodegradable Mg–x Gd implants using small angle X-ray scattering and X-ray diffraction Acta Biomaterialia 101 637–645
Boyde A (2019) Scanning Electron Microscopy of Bone in: Aymen I. Idris (ed.), Bone Research Protocols, Methods in Molecular Biology, vol. 1914, https://doi.org/10.1007/978-1-4939-8997-3_31, © Springer Science+Business Media, LLC, part of Springer Nature 2019
Rousselle S D , Wicks J R, Tabb B C, Tellez A, O’Brien M (2019) Histology Strategies for Medical Implants and Interventional Device Studies Toxicologic Pathology Vol. 47(3) 235-249
Neuerburg C, Mittlmeier L M, Keppler A M, Westphal I, Glass Ä, Saller M M, Herlyn P K E, Richter H, Böcker W, Schieker M, Aszodi A, Fischer D C (2019) Growth factor-mediated augmentation of long bones: evaluation of a BMP-7 loaded thermoresponsive hydrogel in a murine femoral intramedullary injection model. Journal of Orthopaedic Surgery and Research 14 297
Kunert-Keil C, Richter H, Zeidler-Rentzsch I, Bleeker I, Gredes T (2019) Histological comparison between laser microtome sections and ground specimens of implant-containing tissues. Annals of Anatomy 222 153–157
Gabler C, Saß JO, Gierschner S, Lindner T, Bader R, Tischer T (2018) In Vivo Evaluation of Different Collagen Scaffolds in an Achilles Tendon Defect Model. BioMed Research International 208
Wolkers W, Vásquez-Rivera A, Oldenhof H, Dipresa D, Goecke T, Kouvaka A, Will F, Haverich A, Korossis S, Hilfiker A (2018) Use of sucrose to diminish pore formation in freeze-dried heart valves. Scientific Reports 8 12982
Albers, S. Pacilé, M. A. Markus, M. Wiart, G. Vande Velde, G. Tromba, C. Dullin (2018) X-ray-based 3D virtual histology—Adding the next dimension to histological analysis. Mol Imaging Biol (2018) DOI: 10.1007/s11307-018-1246-3
Albers J, Markus MA, Alves F, Dullin C (2018) X-ray based virtual histology allows guided sectioning of heavy ion stained murine lungs for histological analysis. Scientific Reports 8(1) 7712
Boyde A (2018) Evaluation of laser ablation microtomy for correlative microscopy of hard tissues. Journal of Microscopy 271(8) 1-14
Pobloth AM, Checa S, Razi H, Petersen A, Weaver JC, Schmidt-Bleek K, Windolf M, Tatai AÁ, Roth CP, Schaser KD, Duda GN, Schwabe P (2018) Mechanobiologically optimized 3D titanium-mesh scaffolds enhance bone regeneration in critical segmental defects in sheep. Science Translational Medicine 10 423
Joner M, Nicol P, Rai H, Richter H, Foin N, Ng J, Cuesta J, Rivero F, Serrano R, Alfonso F (2018) Very Late Scaffold Thrombosis: Insights from Optical Coherence Tomography and Histopathology. EuroIntervention 13(18)
Boyde A, Staines KA, Javaheri B, Millan JL, Pitsillides AA, Farquharson C (2017) A distinctive patchy osteomalacia characterises Phospho1 deficient mice. Journal of Anatomy 231 298-308
Kowtharapu BS, Marfurt C, Hovakimyan M, Will F, Richter H, Wree A, Stachs O, Guthoff RF (2017) Femtosecond laser cutting of human corneas for the subbasal nerve plexus evaluation. Journal of Microscopy 265(1) 21–26
Daisy Bourassa, Sophie-Charlotte Gleber, Stefan Vogt, Chong Hyun Shinc, Christoph J. Fahrni (2016) MicroXRF tomographic visualization of zinc and iron in the zebrafish embryo at the onset of the hatching period. Metallomics 2016 (8) 1122
Will F, Richter H (2015) Laser-based Preparation of Biological Tissue. Laser Technik Journal 12(5) 44-47
Richter H, Ratliff J, Will F, Stolze B (2015) Time- and material saving laser microtomy for hard tissue and implants. European Cells and Materials 29 Suppl.2 4
Richter H, Ramirez Ojeda DF, Will F (2014) Lasergesteuerte Probenpräparation von Hartgeweben und Biomaterialien. BIOspektrum 05 14
Bourassa D, Gleber S-C, Vogt S, Yi H, Will F, Richter H, Shin CH, Fahrni CJ (2014) 3D Imaging of Transition Metals in the Zebrafish Embryo by X-ray Fluorescence Microtomography. Metallomics 6 1648-1655
Schimek K, Busek M, Brincker S, Groth B, Hoffmann S, Lauster R, Lindner G, Lorenz A, Menzel U, Sonntag F, Walles H, Marx U, Horland R. (2013) Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip 13 3588-3598
Richter H, Ratliff J (2012) A Non-Contact Method of Sectioning Cardiovascular Arteries Containing Metallic Stents Using Laser Technology. J Histotechnol 35 (4) 205
Richter H, Lubatschowski H, Will F (2011) Laser in Medizin & Biologie: Laser-Mikrotomie mit ultrakurzen Pulsen – Neue Perspektiven für die Gewebe- und Biomaterialbearbeitung. Biophotonik 09 50-52
Lubatschowski H, Will F, Przemeck S, Richter H (2011) Laser Microtomy. Handbook of Biophotonics Vol. 2: Photonics for Health Care Wiley-VCH 151-157
Will F, Block T, Menne P, Lubatschowski H (2007) Laser Microtome: all optical preparation of thin tissue samples. Proceedings of SPIE 6460 646007-1
Lubatschowski H (2007) Laser Microtomy – Opening a new Feasibility for Tissue Preparation. Optic & Photonic WILEY-VCH 49 – 51
Menne P (2007) Microtomy with Femtosecond Lasers. Biophotonics International; Laurin Publishing Co. Inc. May 2007 35 – 37
Donath, Sören, Leon Angerstein, Lara Gentemann, Dominik Müller, Anna E. Seidler, Christian Jesinghaus, André Bleich, Alexander Heisterkamp, Manuela Buettner, and Stefan Kalies. (2022). “Investigation of Colonic Regeneration via Precise Damage Application Using Femtosecond Laser-Based Nanosurgery” Cells 11, no. 7: 1143. https://doi.org/10.3390/cells11071143
Müller, Dominik, Sören Donath, Emanuel G. Brückner, Santoshi Biswanath Devadas, Fiene Daniel, Lara Gentemann, Robert Zweigerdt, Alexander Heisterkamp, and Stefan M.K. Kalies. (2021). “How Localized Z-Disc Damage Affects Force Generation and Gene Expression in Cardiomyocytes” Bioengineering 8, no. 12: 213. https://doi.org/10.3390/bioengineering8120213
MoreMüller D, Klamt T, Gentemann L, Heisterkamp A, Kalies SMK (2021) Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes. PLoS ONE 16(6): e0252346. https://doi.org/10.1371/journal.pone.0252346
Baumgart J, Bintig W, Ngezahayo A, Lubatschowski H, Heisterkamp A (2010) Fs-laser-induced Ca2+ concentration change during membrane perforation for cell transfection. Optics Express 18 (3) 2219
Kütemeyer K, Baumgart J, Lubatschowski L, Heisterkamp A (2009) Repetition rate dependency of low density plasma effects during femtosecond-laser-based surgery of biological tissue. Appl. Phys. B 97(3) 695
Baumgart J, Kuetemeyer K, Bintig W, Ngezahayo A, Ertmer W, Lubatschowski H, Heisterkamp A (2009) Repetition rate dependency of reactive oxygen species formation during femtosecond laser-based cell surgery. J Biomed Opt 14(5) 054040
Baumgart J, Bintig W, Ngezahayo A, Willenbrock S, Murua Escobar H, Ertmer W, Lubatschowski H, Heisterkamp A (2008) Quantified femtosecond laser based opto-perforation of living GFSHR-17 and MTH53a cells. Opt. Express 16(5) 3021-3031
Baumgart J, Kuetemeyer K, Bintig W, Ngezahayo A, Ertmer W, Lubatschowski H, Heisterkamp A (2008) Investigation of reactive oxygen species in living cells during femtosecond laser based cell surgery. Proc. SPIE Optical Interactions with Tissue and Cells XIX Vol 6854
Heisterkamp A, Baumgart J, Maxwell IZ, Ngezahayo A, Mazur E, Lubatschowski H (2007) Fs-Laser Scissors for Photobleaching, Ablation in Fixed Samples and Living Cells, and Studies of Cell Mechanics. Laser Manipulation of Cells and Tissues; Elsevier Inc. 293-307