CellSurgeon

High Precision Laser Dissection System

Manipulate and Image – From Organelles To Cells To Tissue To Whole Organisms

CellSurgeon provides a powerful technology platform for precise and gentle laser dissection which enables precise, contact-free 3D-nanodissection of living cells and even subcellular structures. It is a versatile and modular tool suitable for a wide range of applications in biological, medical and pharmaceutical research reaching from subcellular cutting to laser based tissue manipulations in small animal models. Novel experimental approaches to study cellular processes such as cell division, apoptosis, wound healing as well as cell dynamics can be tackled.

Fields of application

  • Dissection of subcellular structures, e.g. cutting of single cytoskeletal filaments
  • Optical knockout or ablation of specific cell organelles
  • Isolation of single cells or cell clusters for further cellular or biochemical analysis in tissues up to small model organisms (e.g. zebrafish embryo)
  • Laser injury models (e.g. vascular, neural)
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Use & Benefits

  • Selective targeting of single cells or cell organelles for manipulation or even destruction
  • In vivo nanosurgery in cells and model organisms from zebrafish to mice
  • Laser dissector and microscopic imaging in one instrument
  • Multiphoton Imaging
  • Time lapse observation after manipulation
  • Combinable with different microscopic techniques (e.g. confocal fluorescence microscopy, multiphoton microscopy, intravital microscopy, spinning disc)

Application Examples

Experiments of accurate cutting in living small organisms with subsequent time-lapse recordings in 3D can easily be performed by means of CellSurgeon

Intracellular cutting and ablation

Cutting of Cellular Microfilaments

Cutting actin filament bundles with a femtosecond laser, in collaboration with R. Schade, Institute for Bioprocessing and Analytical Measurement Techniques, Heiligenstadt, Germany

Subcellular Dissection of Mitotic Spindles

Subcellular Dissection mitotic spindles

GFB-labelled mitotic spindles (fission yeast Schizosaccharomyces pombe), bleaching (A) and cutting/ ablation (B) © I. Raabe, MPI of Cellular Biology & Genetics, Dresden

Cell Organelle Ablation

Mitochondria ablation

Ablation of nucleus with different laser power parameters, before laser ablation (A) and after (B)

Mitochondria ablation

Ablation of mitochondria, before laser ablation (left) and after (right)

Laser injury model in vivo

Thrombus formation after injury

 

In vivo injury of mouse blood vessel by ultrashort pulse laser, brightfield

3D-reconstruction of thrombosis

 

Multiphoton 3D-reconstruction of an in vivo laser-induced thrombus in mouse small vessels

2-Photon-monitoring of blood vessel

 

2-Photon-Imaging of temporal development of clot formation in mouse blood vessels after laser injury @30fps, FITC-Dextran staining

Principle of Laser Nanodissection

An near-infrared ultrashort pulse laser is coupled into a microscope The ultra-short laser pulses (fs-pulses) are focused by a high numerical aperture objective. The high intensities created only within a minimal focal volume cause multiphoton absorption and enable visualization and accurate manipulation of cell structures at a subcellular level in a very precise manner in living cells. Due to virtually no thermal or mechanical energy transfer, the cellular structures adjacent to the tight focus of the laser beam remain unharmed and intact.

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For more information, please have a look at our product brochure