LLS ROWIAK LaserLabSolutions Newsletter

2012-05-29

Newsletter 2/2012

Hyaluronic Acid (HA) Scaffold used as implant for tissue regeneration. Transmitted light (left) and phase contrast (right) (thin section of 10 µm, embedded in epoxy resin)

Dear Scientist,

Explore new possibilities in material and biomaterial sciences to prepare materials for research and analysis with the ROWIAK TissueSurgeon:

  • Is your polymer too hard to prepare thin sections for material analysis?
  • Are ground sections too laborious and time consuming, or no alternative?
  • Or is your material even too soft to be cut with common microtomes?
  • Does a blade of a sledge microtome influence the inner structure of your sample?

Microtomy with an ultrashort pulsed laser could be your solution - Enjoy the Newsletter!



Polyoxymethylene (POM) in polarized light, sectioned with TissueSurgeon (thin section of 10 µm, not embedded)
Polyphtalamide (PPA) in polarized light, sectioned with the TissueSurgeon (thin section of 10 µm, not embedded)

The ROWIAK GmbH adapted the well-established TissueSurgeon from the life sciences area for material sciences to cut non-metal materials with femtosecond lasers. First of all tissues containing biomaterials e.g. scaffolds were cut successfully with the TissueSurgeon. That was the reason to use the technology for polymers from industrial material sciences.

Sectioning polymers and other materials was the next logical step. See the list of materials cut successfully with the ROWIAK TissueSurgeon:

  • Hydrogels
  • Polyoxymethylene (POM)
  • Polyphtalamide (PPA)
  • Polyphenylene Sulfide (PPS)
  • Polyester fibers
  • Liquid Silicone Rubber (LSR)
  • Polytetrafluorethylene (Teflon; PTFE)
  • Diamonds

Scaffolds:

  • cartilage Derived Morphological Proteins (cDMP)
  • Hyaloronic Acid (HA)
  • Polymers (e.g. polycapronate)
  • Tri-Calcium-Phosphate g2 (TCP g2)

Don't see your special material?

Please contact our Product Specialist Dr. Heiko Richter.



ROWIAK at NSH Hard Tissue Forum 2012 in Bethesda, MD

Enamel-Dentin junction of human, non-decalcified tooth, phase contrast (thin section of 20 µm, epoxy resin embedded)

Visit us at

Application & Practice of Hard Tissue Histology Forum
August 18, 2012 in Bethesda, MD, USA

lead by Jack L Ratliff, BA
Owner, Ratliff Histology Consultants
Chairman, Hard Tissue Committee - National Society for Histotechnology (NSH)

You are welcome to get further information about the ROWIAK TissueSurgeon. We are pleased to invite you to the ROWIAK live demonstration. The TissueSurgeon will be available prior to the Hard Tissue Forum from 14th to 17th for hands-on sessions. The Hard Tissue Forum and the demonstration sessions will take place at the Bethesda Doubletree by Hilton.

Arrange your appointment for a demonstration in advance with our Product Specialist Dr. Heiko Richter.



Never Say Never: Sectioning Tissue Samples with Metal Implants

Histological section of murine skin sample. Note the implant tissue interface. The location of the implant shows a gap (*). (thin section of 10 µm, MMA-embedded)

The sectioning of tissue including large metal implants is a challenge in histology. The TissueSurgeon is not able to cut metal samples but it is possible to cut around metal, e.g. metal implants as blood vessels with stents.

As most scaffold materials are very hard but also very brittle, common microtomy is difficult to use. Ground sections are very laborious and much material is lost.
The ROWIAK TissueSurgeon offers a new and easy way to produce serial sections of

  • Materials & Scaffolds without artifacts that appear in common microtomy
  • Soft tissue as the implant-tissue-interface, the area of interest, will be preserved with this method

Read more...



ROWIAK at World Biomaterials Congress 2012 in Chengdu, China

Meet us at

9th World Biomaterials Congress
June 1-5, 2012, Chengdu, China

You get to know the ROWIAK TissueSurgeon and the world’s first biaxial bioreactor TisXell of our co-operation partner QuinXell Technologies Pte Ltd.

Visit ROWIAK GmbH and QuinXell Technologies Pte Ltd at booth No 16, Area B.



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LLS ROWIAK LaserLabSolutions GmbH, Garbsener Landstraße 10, D-30419 Hannover, HRB 210486 , Phone: +49 511 277 2976, Fax: +49 511 277 1766