TissueSurgeon

Non-Contact Precise Cutting of Native Tissue and Material

The TissueSurgeon enables precise and contact free cutting for biological samples, tissue-engineering and material sciences. Based on femto-second laser technology, it can be used for sectioning, structuring or gentle extraction of samples and materials in 2D and 3D for analysis. For navigation and imaging of samples, the TissueSurgeon can be equipped with Optical Coherence Tomography (OCT) and Multiphoton Microscopy (MPM).

  • Cutting of native tissue without time-consuming sample preparation, fixation or cryogenic preparation
  • Cutting of hard tissues such as bone and tooth without decalcification
  • Cutting without contamination for molecular analysis
  • Non-contact cutting, ablating and structuring of biological tissues and materials
  • 3D extraction along implant interfaces for molecular biology (RNA, DNA, proteins)
  • 3D cutting is guided by imaging systems, e. g. Optical Coherence Tomography, Multiphoton Microscopy
  • Precision: 1 µm
  • Available with an integrated OCT imaging system for 3D navigated cutting

The TissueSurgeon is a multi-purpose sectioning instrument, equipped with a femtosecond laser. It enables non-contact cutting as well as ablating and structuring inside biological tissues and various materials. An integrated camera allows the online-monitoring of the cutting process.

The TissueSurgeon is optionally available with a Navigation Module for three-dimensional imaging of specimens. This add-on module is based on Optical Coherence Tomography and enables 3-D navigated cutting. Laser microtomy with integrated OCT imaging technology provides a unique combination of three-dimensional imaging and cutting, faciliating analysis and dissection of samples.

A cost- and time-saving benefit of the TissueSurgeon is the ability to slice different biological tissue in its native state. Extensive and time-consuming sample preparation techniques are not required. In particular the TissueSurgeon is an optimal solution for sectioning tissue which is sensitive to chemical fixation. Even sectioning of hard tissues such as bone and tooth is possible without decalcification. Depending on the material being processed, slice thicknesses of 7 to 100 µm are feasible.

Main component of the TissueSurgeon is a femtosecond laser, tight focussed into the specimen by a high-numerical aperture objective. The high intensities inside the focal volume lead to nonlinear absorption processes and finally to the disruption of the illuminated material. This effect is limited to the focal diameter of about 1 µm. To produce tissue slices both the laser beam and the specimens are moved simultaneously – the laser beam by a fast scanner and the specimen by a piezo driven positioning stage.

The TissueSurgeon is suited for applications in life science as well as in microtechnology and material science.

The gentle extraction of cell material for RNA-analysis out of non-decalcified hard tissue with the ROWIAK TissueSurgeon
3D sectioning of pericardium with the ROWIAK TissueSurgeon
Histology of hard tissues sectioned with the ROWIAK TissueSurgeon
Histology of brain sectioned with the ROWIAK TissueSurgeon

[For more information please click on images]

Laser Parameters

  • Output power: between 2,5 W and 6,0 W available
  • Repetition rate: 10 MHz
  • Pulse duration: 300 fs
  • Wavelength: 1030 nm

Technical Parameters

  • Section thickness: ca. 7 µm – 100 µm
  • Cutting speed: 1 mm² / s
  • Working area: 25 x 35 mm² (larger on request)
  • Dimensions: 1000 x 380 x 810 mm (Y x X x D)
  • Weight: ca. 400 kg

OCT System Parameters

  • Central wavelength: 900 nm +/- 5 nm
  • Spectral bandwidth: 140 nm +/-5 nm
  • A-scan freqency: ~ 5 kHz
  • Optical power: ~ 1.5 mW
  • Image speed: ~ 1 fps (typically)
  • Image depth: max. 1.1 mm
  • Image size: max. 1024 x 512 px
  • Axial resolution: 4.5 µm

MPM-System Parameters

  • Laser for cutting is used for MPM (no additional laser needed; see specification above)
  • Image speed: ~ 1 fps
  • Image depth: max. 400 µm
  • Image size: max. 890 x 890 µm
  • Filter wavelength: SHG (515 nm); THG (343 nm), others on request

Product Brochures & Application Sheets

Related Material

Images

Polymers

Contact

Dr. Heiko Richter
Phone +49 (0)511 277 2952
Fax +49 (0)511 277 1766
h.richter(at)lls-rowiak.de

LLS ROWIAK LaserLabSolutions Worldwide

LLS ROWIAK LaserLabSolutions has distributors around the world to give you the best support. Choose the country and find your local contact:

Belgium

Odyscience sprl
Rue du Vertbois, 11

4000 Liege
Phone +32 (0)4 230 12 22
www.odyscience.com
info(at)odyscience.com

Bulgaria

APEL LASER S.R.L.
nr. 25, Strada Vănâtorilor
077135, Mogosoaia, ILFOV, Romania
Tel./Fax.:  (+40) 213.170.910;  
www.apellaser.ro
alin.enache(at)apellaser.ro

China

Quantum Design China
Room 0306-0308, Air China Plaza
No. 36 Xiaoyun Road, Chaoyang District
Beijing 100027
P. R. China
Tel: +86-10-8512 0277/78/79/80
Fax: +86-10-8512 0276
info(at)qd-china.com
www.qd-china.com

Denmark

A/S Ninolab
Værkstedsvej 24C
4600 Koge
Phone +45 441 1007
Fax +45 4491 1027
Cell +45 2924 0812
tri(at)ninolab.dk
www.ninolab.dk 

France

Odyscience sprl
Rue du Vertbois, 11
4000 Liege, Belgium
Phone +32 (0)4 230 12 22
www.odyscience.com
info(at)odyscience.com

Great Britain / United Kingdom

Auto Q Biosciences Ltd
1210 Parkview
Arlington Business Park
Theale, Berkshire
RG7 4TY
Phone 0118 963 5881
Fax 0118 963 5882
www. autoqbiosciences.com
info(at)autoqbiosciences.com

Ireland

Auto Q Biosciences Ltd
1210 Parkview
Arlington Business Park
Theale, Berkshire
RG7 4TY
Phone 0118 963 5881
Fax 0118 963 5882
www. autoqbiosciences.com
info(at)autoqbiosciences.com

Japan

Kyodo International Inc.
2-10-9 Miyazaki, Miyamae-ku, Kwasaki-shi
Kanagawa-ken, 216-0033, Japan
Phone +81 044 853 2611
Fax +81 044 854 1979
shimokihara(at)yodo-inc.co.jp
www.kyodo-inc.co.jp/english

Korea

GBS,Inc.
#303 SK Twintech Tower B, 345-9
Gasan-dong, Geumcheon-gu
Seoul 153-773
Phone 02-2243-9090
Mobile 010-8677-2349
Fax 02-3391-2342
gmskenny(at)gmail.com
www.gbskr.com

Portugal & Spain

M.T.Brandão,Lda
Rua de Serralves, 599
4150-708 Porto
Phone +351 22 616 73 70
teresa.pintodasilva(at)mtbrandao.com
www.mtbrandao.com

Romania

APEL LASER S.R.L.
nr. 25, Strada Vănâtorilor
077135, Mogosoaia, ILFOV, Romania
Tel./Fax.:  (+40) 213.170.910;  
www.apellaser.ro
alin.enache(at)apellaser.ro

Singapore

Quintech Scientific (Pte) Ltd
No. 9 Chin Bee Avenue 
Singapore 619933
Phone +65 6376 2093
Fax +65 6376 2015
sales(at)quintechscientific.com
www.quintechscientific.com 

Sweden

ab ninolab
Box 137
SE-194 22 Upplands Väsby
Phone +46 (0)8 590 96200
Fax +46 (0)8 590 96210
info(at)ninolab.se
www.ninolab.se 

Taiwan

Sunray Science Co., Ltd.
No. 30, Lane 155, Sanmin Rd.
105, Taipei
Phone (02) 2764-0999
Mobile 0935-725213
Fax (02) 2764-1465
Michael(at)srs.com.tw
www.srs.com.tw

USA

US-Representation
Jim Accuntius
605 Cielo Drive, Georgetown
TX 78628
Phone (512) 639 2897
j.accuntius(at)lls-rowiak.com

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